I.U.B.:3.4.24.3
CollagenaseRawMaterialCertification
Crudecollagenasepreparationscontainseveralisoformsoftwodifferentcollagenases,asulfhydrylprotease,clostripain,atrypsin-likeenzyme,andanaminopeptidase.Thiscombinationofcollagenolyticandproteolyticactivitiesiseffectiveatbreakingdownintercellularmatrices,theessentialpartoftissuedissociation.OnecomponentofthecomplexisahydrolyticenzymewhichdegradesthehelicalregionsinnativecollagenpreferentiallyattheY-GlybondinthesequencePro-Y-Gly-Pro,whereYismostfrequentlyaneutralaminoacid.ThiscleavageyieldsproductssusceptIBLetofurtherpeptidasedigestion.Crudecollagenaseisinhibitedbymetalchelatingagentssuchascysteine,EDTAoro-phenanthrolinebutnotDFP.Itisalsoinhibitedbyα2-macroglobulin,alargeplasmaglycoprotein.Ca2+isrequiredforenzymeactivity.Particularenzymaticprofilesofeachcollagenasehavebeencorrelatedwiththetissuesfromwhichthecellsforstudywereobtained(orwiththeusestowhichthecellsareput)andasaresultofthecorrelationsseveraltypesofcrudecollagenaseshavebeenestablishedbyWorthington:Types1,2,3,and4.
•Type1crudecollagenasehastheoriginalbalanceofcollagenase,caseinase,clostripainandtrypticactivities.
•Type2containshigherrelativelevelsofproteaseactivity,particularlyclostripain.
•Type3containslowestlevelsofsecondaryproteases.
•Type4isdesignedtobeespeciallylowintrypticactivitytolimitdamagetomembraneproteinsandreceptors.
•Purifiedcollagenase,Code:CLSPA,containsminimalsecondaryproteolyticactivitiesalongwithhighcollagenaseactivity.
NEW!AnimalOriginFreeTypesAFA,AFBandAFC,STZ1,andSTZ2collagenasesarederivedfromculturesgrowninmediumcompletelydevoidofanimalbasedcomponentsanddesignedforbioprocessingapplicationswhereintroductionofpotentialanimalderivedpathogensmustbeprevented.LevelsofsecondaryproteasesaresimilartoTypes1and2collagenase. • CLSAFA istheoriginalAFAgradedesignedtohavecollagenaseandsecondaryproteasessimilartoTypes1and2collagenase.• CLSAFB containshighercollagenaseandcaseinaseactivitiesthanCLSAFA. • CLSAFC hasespeciallylowtrypticactivitysimilartoType4collagenase. • STZ1&STZ2,0.22μfilteredSTEMxyme®AOFCollagenase/NeutralProtease(Dispase®)blendsforprimaryandstemcellisolation. |
Worthingtonalsooffers0.22µmfilteredpreparationsofeachtypeinpre-packagedformfordirectreconstitutionanduseincellisolationandcultureprocedures.Correlationsbetweenenzymetypeandeffectivenesswithdifferenttissueshavebeengood,butnotperfect,dueinparttothevariableparametersofuse.Neverthelessmostresearchersconsiderthetissue-typingofcrudecollagenaselotstobeavaluableservice.AdetaileddescriptionoftheWorthingtoncollagenaseandcontaminantassayscanbefoundintheWorthingtonEnzymeManual.Inaddition,tissuespecificreferencesanddetailedisolationconditionscanbefoundintheWorthingtonTissueDissociationGuide.
ThecollagenaseassayisamodificationofMandlwhereincollagenaseisincubatedforfivehourswithnativecollagenandtheextentofcollagenbreakdownisdeterminedusingtheMooreandStein,JBC,176,367,(1948)colorimetricninhydrinmethod.Aminoacidsreleasedareexpressedasmicromolesleucinepermilligramcollagenase.
Uses:Crudecollagenasesarewidelyusedinenzymaticprimarycellisolationandtissuedissociationprocedures.MostresearchersemployeithercrudecollagenasepreparationssuchasTypes1,2,3,and4orchromatographicallypurifiedcollagenase(Code:CLSPA);thelatterusuallycombinedwithsecondaryenzymessuchaselastase,hyaluronidase,etc.Forbestresults,theprecisemixtureofproteolyticactivitiesmustbetailoredtothetissuetobedissociated.Correlationsbetweentypeandeffectivenesswithdifferenttissueshavebeengood,butnotperfect,andmaybedependentpartlyonparametersofuseandobjectivesaswellaslot-to-lotvariations.FormoreinformationseetheCollagenaseSamplingPrograminformation.WorthingtonalsopublishesaTissueDissociationGuidewhichprovidesadditionalinformationregardingtheenzymesusedfortheseapplicationsandspecificreferencesfornumerouscellandtissuetypes.
UnitDefinition:OneUnitreleasesonemicromoleofL-leucineequivalentsfromcollagenin5hoursat37°C,pH7.5
RelatedProducts:
Collagen(CL)
DeoxyribonucleaseI(DP/D/DCLS/D2/DPFF/DPRF)
Elastase(ES/ESL/ESFF)
Hyaluronidase(HSE/HSEP)
NeutralProtease(Dispase,NPRO)
Papain(PAP/PAPL/PAP2)ProteinaseK(PROK)
Trypsin(TL/TRL/TRL3/TRLS/TRTPCK)
TrypsinInhibitors(LBI/OI/SI/SIC)
CellIsolationOptimizingSystem(CIT)
HepatocyteIsolationSystem(HIS)
NeonatalCardiomyocyteIsolationSystem(NCIS)
PapainDissociiationSystem(PDS/PDS2)
Worthington于1947年创办于美国,是一家通过的ISO9001认证的家族企业。 主要生产用于生物技术和生命科学研究,诊断,生物制药和生物加工应用的高质量纯化酶,蛋白质,核酸和试剂盒。我们为所有类型的生命科学应用生产提供优质产品的声誉是70多年经验的结晶。Worthington的内部全方位服务能力包括初始提取,发酵,蛋白质纯化,冻干和包装。所有流程都有完整的文件记录,可从原材料到最终装运进行追溯。
专注领域:
l 酶
l 蛋白质
l 核酸
l 细胞分离试剂盒
l 无动物源性的酶/蛋白质
作为世界上最主要的酶类供应商,以及细胞分离酶的专家级公司,公司的核心酶产品包括:
l 胶原蛋白酶
l 脱氧核糖核酸酶I
l 弹性蛋白酶
l 木瓜蛋白酶
货号 | 产品名称 | 规格 |
LS004194 | Collagenase, Type 1 | 100 mg |
LS004196 | Collagenase, Type 1 | 1 gm |
LS004197 | Collagenase, Type 1 | 5 gm |
LS004174 | Collagenase, Type 2 | 100 mg |
LS004176 | Collagenase, Type 2 | 1 gm |
LS004177 | Collagenase, Type 2 | 5 gm |
LS004180 | Collagenase, Type 3 | 100 mg |
LS004182 | Collagenase, Type 3 | 1 gm |
LS004183 | Collagenase, Type 3 | 5 gm |
LS004186 | Collagenase, Type 4 | 100 mg |
LS004188 | Collagenase, Type 4 | 1 gm |
LS004189 | Collagenase, Type 4 | 5 gm |
LS003126 | Papain, Suspension | 100 mg |
LS003127 | Papain, Suspension | 1 gm |
LS003118 | Papain, Lyophilized | 25 mg |
LS003119 | Papain, Lyophilized | 100 mg |
LS002138 | Deoxyribonuclease I | 25 mg |
LS002139 | Deoxyribonuclease I | 100 mg |
LS002140 | Deoxyribonuclease I | 1 gm |
LS006363 | Elastase, Purified | 5 mg |
LS006365 | Elastase, Purified | 20 mg |
LS003570 | Trypsin Inhibitor, Soybean, Purified | 100 mg |
LS003571 | Trypsin Inhibitor, Soybean, Purified | 1 gm |
LS02100 | Neutral Protease (Dispase), Purified | 10 mg |
LS02104 | Neutral Protease (Dispase), Purified |