I.U.B.:3.1.21.1 

BovinepancreaticdeoxyribonucleaseIproducedrecombinantlyinyeast,Pichiapastoris,todecreaselevelsofcontaminatingRNaseandeliminatepotentialpathogensassociatedwithanimalbasedmaterials.

BovinepancreasisarichsourceofRNaseAwhichisoftenfoundinmanycommercialDNasepreparations.ProducingDNaseIbyrecombinantmeansinanorganismwithmuchlowerlevelsofendogenousRNasegreatlyfacilitatespurificationofanenzymewithundetectablelevelsofRNase.TheprocessesinvolvedintheproductionandisolationofrecombinantDNaseIarecompletelydevoidofanimalbasedcomponentswhicheliminatesthepossibilityofintroducinganimalderivedpathogensintobioprocessingprocedures.

RecombinantDNaseIissuitableforsuchapplicationsas:

•RemovinggenomicDNAfromRNApreparationspriortoRT-PCR

•DegradationofDNAtemplatesaftertranscriptionreactions

•RemovingunwantedDNAfromsamplespriortoNorthernblotting

•RemovingDNAduringbiopharmaandbioprocessingprocedures

UnitDefinition:OneUnitcausesanincreaseinabsorbanceat260nmof0.001perminuteat25^oCwhenactinguponhighlypolymerizedDNAatpH5.0,whichisthesameasotherWorthingtonDNaseIproducts.

Note:Kunitzunitsasreportedbyothersupplierscanbe2to4timeshigherthanKunitzunitsasmeasuredatWorthington.AsmeasuredatWorthington,OneKunitzunitdigests1mgofcalfthymus(orpUC19orl-phage)DNAin10minutesat37^oCin50mMTris,1mMMg²⁺,1mMCa²⁺,pH7.8.CorrelationofdigestionunitswithKunitzunitsmaybedifferentinotherbuffersystems.

StorageBuffer(DR1S):5mMcalciumacetate,4mg/mlglycine,pH5.0and50%glycerol.

DNaseIReactionBuffer(10X):500mMTris-HCl,10mMMgSO₄,1mMCaCl₂,pH7.8,provided.

RelatedProducts:

Albumin,Nuclease-Free(BSANF)
DeoxyribonucleaseII(HDA/HDAC/HDACS)
E•RASERNaseA/T1Blend(RCT)
Histones(H,NHL)
Lysozyme(LY/LYSF) 
Nuclease,Micrococcal(NFCP)
Nuclease,S1(SINUC/SINUCL)
NucleicAcids,DNA,E.coli,LamBDa/Fragments,RNA
Phosphatase,Alkaline(CAP/BAPF/BAPC/BAPSF/PC)
PhosphodiesteraseI(VPH) 
PhosphodiesteraseII(SPH) 
ProteinaseK(PROK/PROKS)
ReverseTranscriptase,RecombinantHIV(RTHIV) 
RibonucleaseA(R/RAF/RASE/RS/RPDF)
RibonucleaseT1,AnimalOriginFree(RT1S)

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