I.U.B.:3.1.21.1

Bovinepancreaticdeoxyribonucleaseisanendonucleasewhichsplitsphosphodiesterlinkages,preferentiallyadjacenttoapyrimidinenucleotideyieldingpolynucleotideswithfreehydroxylgroupatthe3"positionandphosphategroupatthe5"position.Theaveragechainlengthofalimitdigestisatetranucleotide.

Uses:WorthingtonoffersDNaseatdifferentlevelsofpurityfordifferentapplications.ProductCodes:DPRFandDPRFSarebothespeciallydesignedforMolecularBIOLOGyapplicationsandcontainthelowestlevelsofRNaseandproteaseactivity.TheyarebothsuitableforuseintechniquesrequiringdigestionofDNAintherecoveryofintactRNAorwheretheintegrityofstructuralproteinsorenzymesmustbemaintained.Applicationshaveincludednicktranslation,DNAmapping,isolationofnuclearRNAandprotein,plasmidconstruction,andRNApolymerasesynthesisofRNAprobesandRT-PCR.DNaseisalsousedintissuecultureworktodigestDNAfromdamagedcellstherebyreducingviscosity,andremovingmembraneboundDNAfragments.WorthingtonCodes:DPandDCLSaresuitablefortheseapplications.

Stability/Storage:Whenproperlystored,allgradesofWorthingtondeoxyribonucleasearestablefor2-3years.RecommendedstoragetemperatureforallgradesofWorthingtonDNasesis2-8°C.ProductcodeDPRFSmaybestoredat-20°C.Forlongtermstorageinsolution,ProductCodesDandDPFFmaybedissolvedin5mMacetate,1mMcalcium,pH4.5andstoredinsingleusealiquotsat-20°Cor-70°Cforuptooneyear.Onlyfreezeandthawonce;thawedaliquotsarestablerefrigeratedatleastseveralweeks.Additionof50%glycerolwillmaintainaliquidstateat-20°Cwithoutaffectingstabilityandmaterialin50%glycerolcanberemovedandreturnedto-20°Crepeatedly.DPRFisunusuallystableduetotheabsenceofprotease.ForlongtermstorageofDPRFafterreconstitution,usewateroranybufferpH4.0to9.0exceptphosphate;avoidcalciumchelators;add50%glycerolforstorageasliquidat-20°C;aliquotinsingleusecontainers;onlyfreezeandthawonce;thawedaliquotsarestablerefrigeratedatleastseveralweeks.

UnitDefinition:1Unitcausesanincreaseinabsorbanceat260nmof0.001perminutepermlat25°CwhenactinguponhighlypolymerizedDNAatpH5.0.Note:Kunitzunitsasreportedbyothersupplierscanbe2to4timeshigherthanKunitzunitsasmeasuredatWorthington.AsmeasuredatWorthington,oneKunitzunitdigests1µgoflamBDaDNAin10minutesat37°Cin50mMTris,1mMMg²⁺,1mMCa²⁺,pH7.8ina50µlreaction.CorrelationofdigestionunitswithKunitzunitsisdifferentforotherDNAandbuffersystems.

TechnicalNote:ProductCodeDPRF:Eachvialcontainsapproximately2mgglycineand2µmolescalciumper10,000unitsofDNaseI.Dissolvingtheentirevialin5mlprovidestheequivalentofa1mg/mlsolution.

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Worthington于1947年创办于美国，是一家通过的ISO9001认证的家族企业。 主要生产用于生物技术和生命科学研究，诊断，生物制药和生物加工应用的高质量纯化酶，蛋白质，核酸和试剂盒。我们为所有类型的生命科学应用生产提供优质产品的声誉是70多年经验的结晶。Worthington的内部全方位服务能力包括初始提取，发酵，蛋白质纯化，冻干和包装。所有流程都有完整的文件记录，可从原材料到最终装运进行追溯。

专注领域：

l  酶

l  蛋白质

l  核酸

l  细胞分离试剂盒

l  无动物源性的酶/蛋白质

作为世界上最主要的酶类供应商，以及细胞分离酶的专家级公司，公司的核心酶产品包括：

l  胶原蛋白酶

l  脱氧核糖核酸酶I

l  弹性蛋白酶

l  木瓜蛋白酶