The first commercially available collagenase isolated from Clostridium histolyticum was offered by Worthingtonin 1959. At that time we offered one type of crude enzyme which we only tested for collagenase activity.Eventually, with the cooperation of many in the research community, four basic enzyme profiles wereidentified:Type 1 contains average amounts of assayed activities; collagenase, caseinase, clostripain and trypsin. It is generally recommended for adipocytes, epithelial, hepatocytes, lung and adrenal cell isolation. Type 2 contains higher protease contaminant levels, especially clostripain and trypsin. It is generally used for cardiomyocytes, bone, cartilage, muscle, thyroid and endothelial cell isolation.Type 3 contains lower levels of contaminating proteases. It is typically used for mammary and other soft tissues.Type 4 contains especially low trypsin activity. It is typically used for pancreatic islets and other applications where membrane protein and receptor integrity is crucial.CLSPA, Chromatographically purified collagenase, is specially purified to reduce the caseinase activity and provide high specific activity . It is typically used for pancreatic and parotid acini isolation and collagen structural analysis.Worthington also offers 0.22μm filtered collagenase preparations of each Type in 50 mg per vial pre-packaged form for direct reconstitution and use in cell isolation and culture procedures. Correlations between enzyme type and effectiveness with different tissues have been good, but not perfect, due in part to the variable parameters of use. Nevertheless most researchers consider the tissue-typing of crude collagenase lots to be a valuable service. A detailed description of the Worthington collagenase and contaminant assays can be found in the Worthington Enzyme Manual. In addition tissuespecific references and detailed isolation conditions can be found in the Worthington Tissue Dissociation Guide.摘自www.worthington-biochem.com or www.tissuedissociation.com.你可以参考一下这个对你的实验有否帮助~
ahdongyh 2008-10-15