TheWorthingtonNeonatalCardiomyocyteIsolationSystemhasbeendevelopedtoprovideresearcherswithareliable,convenient,andconsistentneonatalratcardiomyocytecellisolationmethod.Byusingpurifiedratherthancrudeenzymepreparations,ithasbeenpossIBLetominimizethelot-to-lotvariation.Inaddition,Worthingtonuse-teststhekitsbyisolatingcardiomyocytesfromneonatalratheartstoassureperformance,reliABIlityandconsistentyieldofviablecells.
ThekithasbeenformulatedincollaborationwithDr.RonalMacGregor.ThemethodisbasedonthatdescribedbyToraason,etal.Toxicol,56,107(1988)inwhichthemincedtissueisincubatedovernightwithpurifiedtrypsinat2-8°C.AspointedoutbyToraason,thisstepreducesthehands-ontimerequiredtoharvestcellscomparedtothetimeinvolvedinsequentialincubationsinwarmtrypsinorCollagenase.Purifiedcollagenaseratherthancrudecollagenaseisusedtomaximizeyieldandviability.
ContentsofKit
Thepackagecontainssufficientmaterialsforfiveseparatetissuedissociations,eachcontaininguptotwelvehearts.Forlargerorsmallertissuesamplesprepareproportionatevolumesofreagentsateachstepandcombinetheminthesameratioasdescribedintheprotocol.
•Vial1:1bottle,500ml:Sterilecalcium-andmagnesium-freeHank"sBalancedSaltSolution(CMFHBSS),pH7.4.ThesolutionisusedforreconstitutingthecontentsofVials#2and#3inadditiontoservingasthemediumforthedissociation.
•Vial2:5vials,1000µgeach:WorthingtonTrypsin(Code:TRLS),3Xcrystallized,dialyzedagainst1mMHCl,filteredthrough0.22micronporesizemembrane,andlyophilized.Beforeuse,reconstitutewith2mlCMFHBSS(Vial#1)andswirlgentlytodissolvecontents.Storeat2-8°C.
•Vial3:5vials,2000µgeach:WorthingtonSoybeanTrypsinInhibitor(Code:SIC),a0.22micronporesizemembranefiltered,lyophilizedpowder.Beforeuse,reconstitutewith1mlCMFHBSS(Vial#1)andswirlgentlytodissolvecontents.Storeat2-8°C.
•Vial4:5vials,1500Unitseach:WorthingtonPurifiedCollagenase(Code:CLSPA),a0.22micronporesizemembranefiltered,lyophilizedpowderwhichhasbeenchromatographicallypurified.Itcontainslessthan50caseinaseunitspermilligramandiscomposedoftwoseparablebutverysimilarcollagenases.Beforeuse,reconstitutewith5mlLeibovitzL-15media(preparedasdescribedbelow)andswirlgentlytodissolvecontents.Storeat2-8°C.
•PouchcontainingLeibovitzL-15MediaPowder:1x1L,Reconstituteentirecontentsofpouchbycuttingopentopofenvelopeandpouringcontentsintobeakercontaining800mlofcellculturegradewater.Rinsepouch2-3timeswithadditional100ml.Bringtotalvolumeto1literandfilterthrougha0.22micronporesizefilter.
Thekitalsoincludes5CellStrainers(Falcon),acardcorrelatingphenolredcolorwithpHforcheckingbalancedsaltsolutionsandculturemedia.
RelatedProducts:
Collagenase(CLS1-4/CLSPA)
DeoxyribonucleaseI(DP/D/DCLS/D2/DPFF/DPRF)
Hyaluronidase(HSE/HSEP)
NeutralProtease(Dispase,NPRO)
Papain(PAP/PAPL/PAP2)
ProteinaseK(PROK)
Trypsin(TL/TRL/TRL3/TRLS/TRTPCK)TrypsinInhibitors(LBI/OI/SI/SIC)
CellIsolationOptimizimgSystem(CIT)
HepatocyteIsolationSystem(HIS)
PapainDissociiationSystem(PDS/PDS2)
Worthington于1947年创办于美国,是一家通过的ISO9001认证的家族企业。 主要生产用于生物技术和生命科学研究,诊断,生物制药和生物加工应用的高质量纯化酶,蛋白质,核酸和试剂盒。我们为所有类型的生命科学应用生产提供优质产品的声誉是70多年经验的结晶。Worthington的内部全方位服务能力包括初始提取,发酵,蛋白质纯化,冻干和包装。所有流程都有完整的文件记录,可从原材料到最终装运进行追溯。
专注领域:
l 酶
l 蛋白质
l 核酸
l 细胞分离试剂盒
l 无动物源性的酶/蛋白质
作为世界上最主要的酶类供应商,以及细胞分离酶的专家级公司,公司的核心酶产品包括:
l 胶原蛋白酶
l 脱氧核糖核酸酶I
l 弹性蛋白酶
l 木瓜蛋白酶
货号 | 产品名称 | 规格 |
LS004194 | Collagenase, Type 1 | 100 mg |
LS004196 | Collagenase, Type 1 | 1 gm |
LS004197 | Collagenase, Type 1 | 5 gm |
LS004174 | Collagenase, Type 2 | 100 mg |
LS004176 | Collagenase, Type 2 | 1 gm |
LS004177 | Collagenase, Type 2 | 5 gm |
LS004180 | Collagenase, Type 3 | 100 mg |
LS004182 | Collagenase, Type 3 | 1 gm |
LS004183 | Collagenase, Type 3 | 5 gm |
LS004186 | Collagenase, Type 4 | 100 mg |
LS004188 | Collagenase, Type 4 | 1 gm |
LS004189 | Collagenase, Type 4 | 5 gm |
LS003126 | Papain, Suspension | 100 mg |
LS003127 | Papain, Suspension | 1 gm |
LS003118 | Papain, Lyophilized | 25 mg |
LS003119 | Papain, Lyophilized | 100 mg |
LS002138 | Deoxyribonuclease I | 25 mg |
LS002139 | Deoxyribonuclease I | 100 mg |
LS002140 | Deoxyribonuclease I | 1 gm |
LS006363 | Elastase, Purified | 5 mg |
LS006365 | Elastase, Purified | 20 mg |
LS003570 | Trypsin Inhibitor, Soybean, Purified | 100 mg |
LS003571 | Trypsin Inhibitor, Soybean, Purified | 1 gm |
LS02100 | Neutral Protease (Dispase), Purified | 10 mg |
LS02104 | Neutral Protease (Dispase), Purified |