I.U.B:3.1.27.3
RibonucleaseT1isanendoribonuclease,highlyspecificforthecleavageofRNAordeaminatedRNAbetweenguanosine3"-phosphateresidues(orinosine3"-phosphate)andthe5"-OHresiduesofadjacentnucleotideswiththeformationofthecorrespondingintermediate2",3"-cyclicphosphates.Itcleavessingle-strandedRNAreleasingoligonucleotidesfromtheguanosine3"-phosphatetermini.Theenzymehasamolecularweightof11kDa.TheoptimumpHis7.5.RNaseT1isinhibitedbyAg+,Zn2+,Cu2+,andHg2+at1X10-3M.ThestimulatoryeffectsofbothhistidineandEDTAareattributedtochelationofcontaminatinginhibitorcations.TheenzymeassayisessentiallythemethodofEgamietal.,Prog.inNucleicAcidRes.andMolec.Biol.,III,59(1964)baseduponthereleaseofacidsolubleoligonucleotidesfollowingthedigestionofyeastRNA.
Uses:RibonucleaseT1hasextensiveapplicationsinmolecularcloningandDNAsequencing.Becauseofitsspecificityithasbeenacommonlyusedcleavageenzymeforthedeterminationofstructure,nearestneighborfrequencies,andRNAsequencing.Theenzymehasfurtherapplicationinthepreparationofnucleoside2",3"-cyclicphosphates,thesynthesisofoligonucleotides,andtheremovalofRNAfromDNApreparations.Theenzymeisalsousedasanon-mammaliansourceofRNaseinvariousapplications.
StABIlity/Storage:Stable12-18monthsat2-8°C.Storeat2-8°C.
UnitDefinition:OneUnitreleasestheequivalentofoneA260unitofacid-solubleproductsfromyeastRNAin15minutesat37°C,pH7.5
TechnicalNote:Somesuppliersreferencesequencingunits;Onesequencingunitisequivalentto0.075Worthingtonunit.
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Worthington于1947年创办于美国,是一家通过的ISO9001认证的家族企业。 主要生产用于生物技术和生命科学研究,诊断,生物制药和生物加工应用的高质量纯化酶,蛋白质,核酸和试剂盒。我们为所有类型的生命科学应用生产提供优质产品的声誉是70多年经验的结晶。Worthington的内部全方位服务能力包括初始提取,发酵,蛋白质纯化,冻干和包装。所有流程都有完整的文件记录,可从原材料到最终装运进行追溯。
专注领域:
l 酶
l 蛋白质
l 核酸
l 细胞分离试剂盒
l 无动物源性的酶/蛋白质
作为世界上最主要的酶类供应商,以及细胞分离酶的专家级公司,公司的核心酶产品包括:
l 胶原蛋白酶
l 脱氧核糖核酸酶I
l 弹性蛋白酶
l 木瓜蛋白酶
货号 | 产品名称 | 规格 |
LS004194 | Collagenase, Type 1 | 100 mg |
LS004196 | Collagenase, Type 1 | 1 gm |
LS004197 | Collagenase, Type 1 | 5 gm |
LS004174 | Collagenase, Type 2 | 100 mg |
LS004176 | Collagenase, Type 2 | 1 gm |
LS004177 | Collagenase, Type 2 | 5 gm |
LS004180 | Collagenase, Type 3 | 100 mg |
LS004182 | Collagenase, Type 3 | 1 gm |
LS004183 | Collagenase, Type 3 | 5 gm |
LS004186 | Collagenase, Type 4 | 100 mg |
LS004188 | Collagenase, Type 4 | 1 gm |
LS004189 | Collagenase, Type 4 | 5 gm |
LS003126 | Papain, Suspension | 100 mg |
LS003127 | Papain, Suspension | 1 gm |
LS003118 | Papain, Lyophilized | 25 mg |
LS003119 | Papain, Lyophilized | 100 mg |
LS002138 | Deoxyribonuclease I | 25 mg |
LS002139 | Deoxyribonuclease I | 100 mg |
LS002140 | Deoxyribonuclease I | 1 gm |
LS006363 | Elastase, Purified | 5 mg |
LS006365 | Elastase, Purified | 20 mg |
LS003570 | Trypsin Inhibitor, Soybean, Purified | 100 mg |
LS003571 | Trypsin Inhibitor, Soybean, Purified | 1 gm |
LS02100 | Neutral Protease (Dispase), Purified | 10 mg |
LS02104 | Neutral Protease (Dispase), Purified |