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培养基参考文献牛牛Adipocytes
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1982(还有鼠, 沙鼠, 兔, 羔羊, 豚鼠)仓鼠仓鼠, 5 周-12 个月White fatCollagenase: 0.05%Kreb s Ringer bicarbonate bufferDiGirolamo, M., Mendlinger, S., and Fertig, J.W.:A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species,Am J Physiol 221, 850, 1971人人Mesenchymal Stromal CellsCollagenase Type 4: 0.2%PBSChoudhery,M, Badowski, M, Muise, A and Harris, D:Effect of Mild Heat Stress on the Proliferative and Differentiative Ability of Human Mesenchymal Stromal Cells.,Cytotherapy17, 359-68, 2015人人Adipose derived stem cellsCollagenase Type 2: 0.1%HBSSSatish, L., Krill-Burger, J., Gallo, P., Etages, S., Liu, F., Philips, B., Ravuri, S., Marra, K., LaFramboise, W., Kathju, S. and Rubin, J.:Expression Analysis of Human Adipose-Derived Stem Cells During In Vitro Differentiation to an Adipocyte Lineage.,BMC Med Genomics8, 41, 2015人人, 女性Adipose StromalCollagenase Type 1: 0.075%DMEMKinoshita, K., Kuno, S., Ishimine, H., Aoi, N., Mineda, K., Kato, H., Doi, K., Kanayama, K., Feng, J., Mashiko, T., 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Phenotypical Characteristics of Human Adipose Tissue-Derived Stem Cells: Comparison of Ficoll Gradient Centrifugation and Red Blood Cell Lysis Buffer Treatment Purification Methods.,Cytotherapy16, 1220-8, 2014人人Adipose stromal stemCollagenase animal free: 200 u/mlDMEM/Hams F-12Carvalho, P., Gimble, J., Dias, I., Gomes, M. and Reis, R.:Xenofree Enzymatic Products for the Isolation of Human Adipose-Derived Stromal/Stem Cells.,Tiss Eng19, 473-8, 2013人人Stromal Vascular FractionCollagenase Type 1: 0.075%PBSDoi, K., Tanaka, S., Iida, H., Eto, H., Kato, H., Aoi, N., Kuno, S., Hirohi, T. and Yoshimura, K.:Stromal Vascular Fraction Isolated from Lipo-Aspirates Using an Automated Processing System: Bench and Bed Analysis.,J Tissue Eng Regen Med7, 864, 2013人人Adipose-derived stem cellsCollagenase Type 1: 0.1%PBSCervelli, V., Scioli, M., Gentile, P., Doldo, E., Bonanno, E., Spagnoli, L. and Orlandi, A.:Plaet-Rich Plasma Greatly Potentiates Insulin-Induced Adipogenic Differentiation of Human Adipose-Derived Stem Cells Through a Serine/Threonine Kinase Akt-dependent Mechanism and Promotes Clinical Fat Graft Maintenance.,Stem Cells Transl Med1, 206-20, 2012人人PerivascularCollagenase Type 2: 0.1%DMEMJames, A., Zara, J., Corselli, M., Askarinam, A., Zhou, A., Hourfar, A., Nguyen, A., Megerdichian, S., Asatrian, G., Pang, S., Stoker, D., Zhang, X., Wu, B., Ting, K., Peault, B. and Soo, C.:An Abundant Perivascular Source of Stem Cells for Bone Tissue Engineering.,Stem Cells Transl Med1, 673, 2012人人Adipose derived stromal vascularCollagenase Type 1: 0.1%PBSGentile, P., Orlandi, A., Scioli, M., Di Pasquali, C., Bocchini, I. and Cervelli, V.:Concise Review: Adipose-Derived Stromal Vascular Fraction Cells and Plaet-Rich Plasma: Basic and Clinical Implications for Tissue Engineering Therapies in Regenerative Surgery.,Stem Cells Transl Med1, 230-6, 2012人人Stromal vascular, adipocytesCollagenase Type 1: 0.1%DMEMGentile, P., Orlandi, A., Scioli, M., Di Pasquali, C., Bocchini, I., Curcio, C., Floris, M., Fiaschetti, V., Floris, R. and Cervell, V.:A Comparative Translational Study: The Combined Use of Enhanced Stromal Vascular Fraction and Plaet-Rich Plasma Improves Fat Grafting Maintenance in Breast Reconstruction.,Stem Cells Transl Med1, 341-51, 2012人人Chorionic mesenchymalCollagenase Type 1: 0.1%MEMNazarov, I., Lee, J., Soupene, E., Etemad, S., Knapik, D., Green, W., Bashkirova, E., Fang, X., Matthay, M., Kuypers, F. and Serikov, V.:Multipotent Stromal Stem Cells from Human Placenta Demonstrate High Therapeutic Potential.,Stem Cells Transl Med1, 359-72, 2012Neutral Protease: 2.5 u/mlTrypsin: 0.25%人人, 成年的StromovascularCollagenase Type 1: 0.1%PBS with BSAHagman, D., Kuzma, J., Larson, I., Foster-Schubert, K., Kuan, L., Cignarella, A., Geamanu, E., Makar, K., Gottlieb, J. and Kratz, M.:Characterizing and Quantifying Leukocyte Populations in Human Adipose Tissue: Impact of Enzymatic Tissue Processing.,J Immunol Methods 386, 50, 2012人人Adipose derived stromalCollagenase Type 1: 0.1%PBSNaaijkens, B., Niessen, H., Prins, H., Krijnen, P., Kokhuis, T., de Jong, N,, van Hinsbergh, V., Kamp, O., Helder, M., Musters, R., van Dijk, A. and Juffermans, L.:Human Plaet Lysate as a Fetal Bovine Serum Substitute Improves Human Adipose-Derived Stromal Cell Culture for Future Cardiac Repair Applications.,Cell Tissue Res348, 119, 2012人人Adipose derived stemCollagenase Type 1: 0.1%DMEM/F-12Wu, I., Nahas, Z., Kimmerling, K., Rosson, G. and Elisseeff, J.:An Injectable Adipose Matrix for Soft-Tissue Reconstruction.,Plast Reconstr Surg129, 1247, 2012人人, 男性 40-60岁Adipose derived stem cellsCollagenase: 0.25%PBSBlasi, A., Martino, C., Balducci, L., Saldarelli, M., Soleti, A., Navone, S., Canzi, L., Cristini, S., Invernici, G., Parati, E. and Alessandri, G.:Dermal Fibroblasts Display Similar Phenotypic and Differentiation Capacity to Fat-Derived Mesenchymal Stem Cells, but Differ in Anti-Inflammatory and Angiogenic Potential,Vasc Cell3, 5, 2011Deoxyribonuclease I: 0.002%人人Renal adipose derived cellsCollagenase Type 1: 0.3%DMEMBasu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.:Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney.,Lipids Health Dis10, 171, 2011人人Adipose derived mesenchymalCollagenase Type 1: 0.1%L-DMEMYang, X., He, X., He, J., Zhang, L., Su, X., Dong, Z., Xu, Y., Li, Y. and Li, YL.:High Efficient Isolation and Systematic Identification of Human Adipose-Derived Mesenchymal Stem Cells.,J Biomed Sci18, 59, 2011人人Adipose derived stemCollagenase Type 1: 0.1%DMEM/Ham s F-12Yu, G. Floyd, ZE., Wu, X., Halvorsen, Y. and Gimble, J.:Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates.,Methods Mol Biol 702, 17-27, 2011人人AdipocytesCollagenase Type 1: 0.1%RPMIBasu, S., Haghiac, M., Surace, P., Challier, J., Guerre-Millo, M., Singh, K., Waters, T., Minium, J., Presley, L., Catalano, P. and Hauguel-de Mouzon, S.:Pregravid Obesity Associates with Increased Maternal Endotoxemia and Metabolic Inflammation.,Obesity19, 476, 2011人人AdipocytesCollagenase Type 1: 0.1%KRBPeters, R., Wolf, M., van den Broek, M., Nuvolone, M., Dannenmann, S., Stieger, B., Rapold, R., Konrad, D., Rubin, A., Bertino, J., Aguzzi, A., Heikenwalder, M. and Knuth, A.:Efficient Generation of Multipotent Mesenchymal Stem Cells from Umbilical Cord Blood in Stroma-Free Liquid Culture.,PLoS ONE5, e15689, 2010人人Adipose tissue-derived stemCollagenase Type 2: 1.0%DMEM/F12Tan, H., DeFail, A., Rubin, J., Chu, C. and Marra, K.:Novel Multiarm PEG-Based Hydrogels for Tissue Engineering.,J Biomed Mater Res92, 979, 2010人人Stromal vascularCollagenase Type 2: 0.1%HBSSZimmerlin, L., Donnenberg, V., Pfeifer, M., Meyer, EM, Peault, B., Rubin, JP and Donnenberg, A.:Stromal Vascular Progenitors in Adult Human Adipose Tissue.,Cytometry 77, 22-30, 2010人人Adipose stromalCollagenase Type 1: 0.1%DMEM/Hams F-12Yu, G., Wu, X., Dietrich, M., Polk, P., Scott, LK, Ptitsyn, A. and Gimble, J.:Yield and Characterization of Subcutaneous Human Adipose-Derived Stem Cells by Flow Cytometric and Adipogenic mRNA Analyzes.,Cytotherapy12, 538, 2010人人AdipocytesCollagenase: 0.075%DMEMSuga, H., Eto, H., Shigeura, T., Inoue, K., Aoi, N., Kato, H., Nishimura, S., Manabe, I., Gonda, K. and Yoshimura, K.:IFATS Collection: Fibroblast Growth Factor-2-Induced Hepatocyte Growth Factor Secretion by Adipose-Derived Stromal Cells Inhibits Postinjury Fibrogenesis Through a c-Jun N-Terminal Kinase-Dependent Mechanism.,Stem Cells 27, 238, 2009人人Adipose Stromal CellCollagenase Type 1: 0.1%DMEMCai, L., Johnstone, B., Cook, T., Tan, J., Fishbein, M., Chen, P. and March, K.:IFATS Collection: Human Adipose Tissue-Derived Stem Cells Induce Angiogenesis and Nerve Sprouting Following Myocardial Infarction, in Conjunction with Potent Preservation of Cardiac Function.,Stem Cells 27, 230, 2009人人Adipose tissue-derived stemCollagenase Type 2: 1.0%DMEM/F12Tan, H., Ramirez, C., Miljkovic, N., Li, H., Rubin, J. and Marra, K.:Thermosensitive Injectable Hyaluronic Acid Hydrogel for Adipose Tissue Engineering.,Biomaterials 30, 6844, 2009人人Adipose derived stem cellsCollagenase Type 1: 0.1%DMEMTandon, N., Goh, B., Marsano, A., Chao, P., Montouri-Sorrentino, C., Gimble, J. and Vunjak-Novakovic, G.:Alignment and Elongation of Human Adipose-Derived Stem Cells in Response to Direct-Current Electrical Stimulation.,Conf Proc IEEE Eng Med Biol Soc 2009, 6517-21, 2009人人Adipose derived stromal cellsCollagenase Type 1: see referenceDMEMTraktuev, D., Merfeld-Clauss, S., Li, J., Kolonin, M., Arap, W., Pasqualini, R., Johnstone, B., and March, K.:A Population of Multipotent CD34-Positive Adipose Stromal Cells Share Pericyte and Mesenchymal Surface Markers, Reside in a Periendothelial Location, and Stabilize Endothelial Networks,Circ Res 102, 77, 2008人人StromalCollagenase Type 1: 0.2%PBSMinana, M., Carbonell-Uberos, F., Mirabet, V., Marin, S. and Encabo, A.:IFATS Collection: Identification of Hemangioblasts in the Adult Human Adipose Tissue.,Stem Cells 26, 2696, 2008人人StromalCollagenase Type 1: 0.1%DMEMNie, J., Chang, B., Traktuev, D., Sun, J., March, K., Chan, L., Sage, H., Pasqualini, R., Arap, W. and Kolonin, M.:IFATS Collection: Combinatorial Peptides Identify alpha-5 beta-1 Integrin as a Receptor for the Matricellular Protein SPARC on Adipose Stromal Cells.,Stem Cells 26, 2735, 2008Neutral Protease:人人Stem CellsCollagenase: 280 u/mlD-PBSPilgaard, L., Lund, P., Rasmussen, J., Fink, T. and Zachar, V.:Comparative Analysis of Highly Defined Proteases for the Isolation of Adipose Tissue-Derived Stem Cells.,Regen Med3, 705-15, 2008人人AdipocytesCollagenase Type 1: 0.2%HBSSBujalska Iwona J, Durrani Omar M, Abbott Joseph, Onyimba Claire U, Khosla Pamela, Moosavi Areeb H, Reuser Tristan T Q, Stewart Paul M, Tomlinson Jeremy W, Walker Elizabeth A, Rauz Saaeha:Characterisation of 11beta-hydroxysteroid dehydrogenase 1 in human orbital adipose tissue: a comparison with subcutaneous and omental fat,J Endocrinol 192, 279-88, 2007人人Adipose derived stromal cellsCollagenase Type 1: 0.15%DMEMSchaffler A, Buchler C:Concise review: adipose tissue-derived stromal cells--basic and clinical implications for novel cell-based therapies,Stem Cells 25, 818-27, 2007人人Adipose derived stem cellsCollagenase Type 1: 0.1%DMEM/F12Kilroy, G., Foster, S., Wu, X., Ruiz, J., Sherwood, S., Heifetz, A., Ludlow, J., Stricker, D., Potiny, S., Green, P., Halvorsen, Y., Cheatham, B., Storms, R. and Gimble, J.:Cytokine Profile of Human Adipose-Derived Stem Cells: Expression of Angiogenic, Hematopoietic, and Pro-Inflammatory Factors.,J Cell Physiol212, 702-9, 2007人人Mesenchymal stemCollagenase Type 1: 0.1%HBSSJeon Eun Su, Song Hae Young, Kim Mi Ra, Moon Hyun Jung, Bae Yong Chan, Jung Jin Sup, Kim Jae Ho:Sphingosylphosphorylcholine induces proliferation of human adipose tissue-derived mesenchymal stem cells via activation of JNK,J Lipid Res 47, 653-64, 2006人人PreadipocytesCollagenase Type 1: 196 u/mlM199Koellensperger, E., Von Heimburg, D., Markowicz, M. and Pallua, N.:Human Serum from Plaet-Poor Plasma for the Culture of Primary Human Preadipocytes.,Stem Cells 24, 1218-25, 2006人人Adipocytes, stromal vascularCollagenase: 0.2%HBSSBoquest Andrew C, Shahdadfar Aboulghassem, Fronsdal Katrine, Sigurjonsson Olafur, Tunheim Siv H, Collas Philippe, Brinchmann Jan E:Isolation and transcription profiling of purified uncultured human stromal stem cells: alteration of gene expression after in vitro cell culture,Mol Biol Cell16, 1131-41, 2005人人Multipotent adipose derived stemCollagenase: 0.2%DMEMRodriguez Anne-Marie, Pisani Didier, Dechesne Claude A, Turc-Carel Claude, Kurzenne Jean-Yves, Wdziekonski Brigitte, Villageois Albert, Bagnis Claude, Breittmayer Jean-Philippe, Groux Herve, Ailhaud Gerard, Dani Christian:Transplantation of a multipotent cell population from human adipose tissue induces dystrophin expression in the immunocompetent mdx mouse,J Exp Med 201, 1397-405, 2005人人成年的 stem cellsCollagenase Type 1: 0.1%PBSDevireddy, R., Thirumala, S. and Gimble, J.:Cellular Response of Adipose Derived Passage-4 Adult Stem Cells to Freezing Stress.,J Biomech Eng 127, 1081, 2005人人, 成年的, 肥胖的AdipocytesCollagenase Type 2: 0.1%DMEM/F12Seboek, D., Linscheid, P., Zulewski, H., Langer, I., Christ-Crain, M., Keller, U., and Muller, B.:Somatostatin Is Expressed and Secreted by Human Adipose Tissue Upon Infection and Inflammation,J Clin Endocrinol Metab 89(10), 4833, 2004人人, 成年的, 女性PreadipocytesCollagenase Type 1: 0.2%DMEM/F-12Quickler, M., Sinha, B., Tomlinson, J., Bujalska, I., Stewart, P., Arlt, W.,:Androgen generation in adipose tissue in women with simple obesity--a site-specific role for 17beta-hydroxysteroid dehydrogenase type 5,J Endocrinol 183, 331, 2004人人,成年的AdipocytesCollagenase Type 1: 0.13%see referenceFain JN, Madan AK, Hiler ML, Cheema P, and Bahouth SW.:Comparison of the release of adipokines by adipose tissue, adipose tissue matrix, and adipocytes from visceral and subcutaneous abdominal adipose tissues of obese humans,Endocrinology 145, 2273, 2004人人Stromal vascular, adipocytesCollagenase: 0.2%DMEM/F12Planat-Benard Valerie, Silvestre Jean-Sebastien, Cousin Beatrice, Andre Mireille, Nibbelink Maryse, Tamarat Radia, Clergue Michel, Manneville Carole, Saillan-Barreau Corinne, Duriez Micheline, Tedgui Alain, Levy Bernard, Penicaud Luc, Casteilla Louis:Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives,Circulation 109, 656-63, 2004人人Stromal vascular, adipocytes, stemCollagenase: 300 u/mlPBSMiranville A, Heeschen C, Sengenes C, Curat CA, Busse R, Bouloumie A:Improvement of postnatal neovascularization by human adipose tissue-derived stem cells,Circulation 110, 349-55, 2004人人, 成年的, 女性, 非糖尿病的AdipocytesCollagenase Type 2: 0.05%DMEMGesta, S., Lolmede, K., Daviaud, D., Berlan, M., Bouloumie, A., Lafontan, M., Valet, P., and Saulnier-Blache, J.:Culture of human adipose tissue explants leads to profound alteration of adipocyte gene expression,Horm Metab Res35, 158, 2003人人AdipocytesCollagenase Type 1: see referenceSalinePatwardhan, R., Tubbs, R., Leonard, R., Kelly, D., Killingsworth, C., Rollins, D., Smith, W., Ideker, R., and Oakes, W.:Discernment of Adipose versus Nervous Tissue: A Novel Adjunct Solution in Lipomyelomeningocele Surgery,Ped Neurosurg 36, 314, 2002人人, 成年的, 男性和女性AdipocytesCollagenase Type 1: 0.2%DMEM/F12McTernan, P., Anderson, L., Anwar, A., Eggo, M., Crocker, J., Barnett, A., Stewart, P., and Kumar, S.:Glucocorticoid Regulation of P450 Aromatase Activity in Human Adipose Tissue: Gender and Site Differences,J Clin Endocrinol Metab 87, 1327, 2002人人Processed lipoaspi鼠e cellsCollagenase Type 1: 0.075%PBSZuk Patricia A, Zhu Min, Ashjian Peter, De Ugarte Daniel A, Huang Jerry I, Mizuno Hiroshi, Alfonso Zeni C, Fraser John K, Benhaim Prosper, Hedrick Marc H:Human adipose tissue is a source of multipotent stem cells,Mol Biol Cell13, 4279-95, 2002人人AdipocytesCollagenase Type 1: 0.2%HBSSMcTernan PG, Anwar A, Eggo MC, Barnett AH, Stewart PM, Kumar S:Gender differences in the regulation of P450 aromatase expression and activity in human adipose tissue,Int J Obes Relat Metab Disord24, 875-81, 2000人人, 成年的, 男性和女性AdipocytesCollagenase Type 1: 0.1%DMEM/Ham s F-12Gottschling-Zelle, H., Birgel, M., Scriba, D., Blum, W., and Hauner, H.:Depot-specific Release of Leptin from Subcutaneous and Omental Adipocytes in Suspension Culture: Effect of Tumor Necrosis Factor-alpha and Transforming Growth Factor-beta1,Eur J Endocrinol 141 (4), 436, 1999人人, 成年的, 男性和女性AdipocytesCollagenase Type 1: 0.1%DMEM/F12Zhang, H., Kumar, S., Barnett, A., and Eggo, M.:Intrinsic Site-Specific Differences in the Expression of Leptin in Human Adipocytes and Its Autocrine Effects on Glucose Uptake,J Clin Endocrinol Metab 84, 2550, 1999人人, 非糖尿病的, 男性AdipocytesCollagenase Type 1: 0.05%Kreb s Ringer bicarbonate bufferAnderson, O., Gliemann, J., and Gammeltoft:Receptor Binding and Biological Effect of Insulin in Human Adipocytes,Diabetologia 13, 589, 1977小鼠小鼠AdipocytesCollagenase: 0.1%KrebsJang, H., Bhasin, S., Guarneri, T., Serra, C., Schneider, M., Lee, M., Guo, W., Fried, S., Pencina, K. and Jasuja, R.:The Effects of A Single Developmentally-Entrained Pulse of Testosterone in Female Neonatal Mice On Reproductive and Metabolic Functions in Adult Life.,Endocrinology, EN20151117, 2015小鼠小鼠, 20 周Stromal VascularCollagenase Type 2: 0.2%RPMI-1640Kondo, T, Toyoshima, Y., Ishii, Y and Kyuwa, S.:Natural Killer T Cells in Adipose Tissue are Activated in Lean Mice.,Exp Anim62, 319, 2013小鼠小鼠Adipose derived stemCollagenase Type 2: 0.1%DMEMTakahashi, H., Haraguchi, N., Nishikawa, S., Miyazaki, S., Suzuki, Y., Mizushima, T., Nishimura, J., Takemasa, I., Yamamoto, H., Mimori, K., Ishii, H., Doki, Y. and Mori, M.:Biological and Clinical Availability of Adipose-Derived Stem Cells for Pelvic Dead Space Repair.,Stem Cells Transl Med1, 803, 2012小鼠小鼠Stem and progenitorCollagenase Type 2: 0.2%HBSSHan, J., Koh, Y., Moon, H., Ryoo, H., Cho, C., Kim, I. and Koh, G.:Adipose Tissue is an Extramedullary Reservoir for Functional Hematopoietic Stem and Progenitor Cells.,Blood 115, 957, 2010小鼠小鼠, 3 周AdipocytesCollagenase Type 1: 0.2%HBSSDe Matteis, R., Zingaretti, M., Murano, I., Vitali, A., Frontini, A., Giannulis, I., Barbali, G., Marcucci, F., Bordicchia, M., Sarzani, R., Raviola, E. and Cinti, S.:In Vivo Physiological Transdifferentiation of Adult Adipose Cells.,Stem Cells 27, 2761, 2009小鼠小鼠White adipocytesCollagenase Type 2: 0.1%DMEMWong, K., Szeto, F, Zhang, W., Ye, H., Kong, J., Zhang, Z., Sun, X. and Li, Y:Involvement of the Vitamin D Receptor in Energy Metabolism: Regulation of Uncoupling Proteins.,Am J Physiol/Endo 296, 820, 2009小鼠小鼠Adipose Stromal CellCollagenase Type 2: 0.075%DMEMThangarajah, H., Vial, I., Chang, E., El-Ftesi, S., Januszyk, M., Chang, E., Paterno, J., Neofytou, E., Longaker, M. and Gurtner, G.:IFATS Collection: Adipose Stromal Cells Adopt a Proangiogenic Phenotype Under the Influence of Hypoxia.,Stem Cells 27, 266, 2009小鼠小鼠Adipose tissue-derived stemCollagenase Type 1: 0.075%Modified EaglesCho, K., Park, H., Park, H., Jung, J., Jeon, S., Kim, Y. and Roh, H.:IFATS Collection: Immunomodulatory Effects of Adipose Tissue-Derived Stem Cells in an Allergic Rhinitis Mouse Model.,Stem Cells 27, 259-65, 2009小鼠小鼠, C57Bl/6JAdipocytesCollagenase Type 1: 0.15%DMEM/F12Aoyagi T, Shimba S, Tezuka M: Characteristics of Circadian Gene Expressions in Mice White Adipose Tissue and 3T3-L1 Adipocytes,J Health Sci 51, 21, 2005小鼠小鼠, 3-6 天 和 2-3 个月Adipose-derived stromal cellsCollagenase Type 2: 0.075%PBSCowan Catherine M, Shi Yun-Ying, Aalami Oliver O, Chou Yu-Fen, Mari Carina, Thomas Romy, Quarto Natalina, Contag Christopher H, Wu Benjamin, Longaker Michael T:Adipose-derived adult stromal cells heal critical-size mouse calvarial defects,Nat Biotechnol22, 560-7, 2004小鼠小鼠Stromal vascular, adipocytesCollagenase: 0.2%DMEM/F12Planat-Benard Valerie, Silvestre Jean-Sebastien, Cousin Beatrice, Andre Mireille, Nibbelink Maryse, Tamarat Radia, Clergue Michel, Manneville Carole, Saillan-Barreau Corinne, Duriez Micheline, Tedgui Alain, Levy Bernard, Penicaud Luc, Casteilla Louis:Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives,Circulation 109, 656-63, 2004小鼠小鼠, C57BL/6J and FVB, 雄性, 8-9 周AdipocytesCollagenase: see referenceDMEMRuan, H., Zarnowski, MJ., Cushman, S., and Lodish, H.:Standared Isolation of Primary Adipose Cells from Mouse Epididymal Fat Pads Induces Inflammatory Mediators and Down-regulates Adipocyte Genes,J Biol Chem278, 47585, 2003小鼠小鼠, 雌雄合计AdipocytesCollagenase: 0.05%Krebs-Ringer Phosphate HEPES (KRPH)Nadler, S., Stoehr, J., Rabaglia, M., Schueler, K., Birnbaum, M., and Attie, A.:Normal Akt/PKB with Reduced PI3K Activation in Insulin-resistant Mice,Am J Physiol/Endo 281, E1249, 2001小鼠小鼠, B6D2F1,杂交子代,新西兰黑 雌性 新西兰黑 雄性Vascular endothelialCollagenase: 0.2%PBSLaunder, T., Gegen, N., Knedler, A., and Harbeck, R.:The Isolation and Characterization of Enriched Microvascular Endothelial Cells From Mouse Adipose Tissue,J Immunol Methods 102, 45, 1987猪猪, 雌性, 一年以下Adipose mesenchymal stemCollagenase Type 1: 0.1%DMEMWilliams, K., Picou, A., Kish, S., Giraldo, A., Godke, R. and Bondioli, K:Isolation and Characterization of Porcine Adipose Tissue-Derived Adult Stem Cells.,Cells Tissues Organs188, 251, 2008猪猪, 杂交繁育, 雄性 1-4 天AdipocytesCollagenase Type 1: 0.2%DMEM/F12Ramsay, T.G.:Porcine Leptin Inhibits Lipogenesis in Porcine Adipocytes,J Anim Sci 81, 3008, 2003猪猪, 杂交繁育的AdipocytesCollagenase Type 1: 0.067%Krebs-RingerLiang, W. and Mills, S.:Quantitative Analysis of beta-Adrenergic Receptor Subtypes in Pig Tissues.,J Anim Sci 80, 963-70, 2002猪猪, 8-9 周AdiposeCollagenase Type 1: 300 u/mlHEPESDing, S., McNeel, R., and Mersmann, H.:Expression of Porcine Adipocyte Transcripts: Tissue Distribution and DifferentiationIn VitroandIn Vivo,Comp Biochem Physiol B123, 307, 1999猪猪, 初生AdipocytesCollagenase Type 1: 0.3%Krebs-Ringer bicarbonate albuminWang Y, Fried SK, Petersen RN, Schoknecht PA:Somatotropin regulates adipose tissue metabolism in neonatal swine,J Nutr 129, 139-45, 1999猪猪, 杂交繁育, 1-3 天Adipose, Stromal-vascularCollagenase Type 1: 0.2%DMEM/F12Suryawan, A., Swanson, L., and Hu, C.:Insulin and Hydrocortisone, But Not Triiodothyronine, Are Required for the Differentiation of Pig Preadipocytes in Primary Culture,J Anim Sci 75, 105, 1997小鼠小鼠Mesenchymal Stem CellsCollagenase Type 1: 0.075%DMEMEmre, E, Yuksel, N, Duruksu, G, Pirhan, D, Subasi, C, Erman, G and Karaoz, E:Neuroprotective Effects of Intravitreally Transplanted Adipose Tissue and Bone Marrow-Derived Mesenchymal Stem Cells in an Experimental Ocular Hypertension Model.,Cytotherapy17, 543-59, 2015小鼠小鼠, SD, 5 个月Mesenchymal Stromal CellsCollagenase Type 2: 0.075%DMEMVeronesi, F, Torricelli, P, Della, B, Pagani, S and Fini, M:In Vitro Mutual Interaction Between Tenocytes and Adipose-Derived Mesenchymal Stromal Cells.,Cytotherapy17, 215-23, 2015大鼠大鼠AdipocytesCollagenase Type 1: 0.3%Krebs-RingerOgasawara, J., Izawa, T., Sakurai, T., Shirato, K., Ishibashi, Y., Ohira, Y., Ishida, H., Ohno, H. and Kizaki, T.:Habitual Exercise Training Acts as a Physiological Stimulator for Constant Activation of Lipolytic Enzymes in Rat Primary White Adipocytes.,Biochem Biophys Res Commun464, 348-53, 2015大鼠大鼠, 成年的Stromal vascularCollagenase Type 1: 0.2%PBSLeblanc, A., Nguyen, Q., Touroo, J., Aird, A., Chang, R., Ng, C., Hoying, J. and Williams, S.:Adipose-derived Cell Construct Stabilizes Heart Function and Increases Microvascular Perfusion in an Established Infarct.,Stem Cells Transl Med2, 896-905, 2013大鼠大鼠, 雄性, 300gAdipocytesCollagenase Type 2: 0.33%RPMI 1640Thompson, A., Nunez, M., Davidson, R., Horm, T., Schnittker, K., Hart, M., Suarez, A. and Tsao, T.:Mitigation of Isolation-Associated Adipocyte Interleukin-6 Secretion Following Rapid Dissociation of Adipose Tissue.,J Lipid Res 53, 2797, 2012大鼠大鼠, 路易斯, 雄性Renal adipose derived cellsCollagenase Type 1: 0.3%DMEMBasu, J., Genheimer, C., Sangha, N., Quinlan, S., Guthrie, K., Kelley, R., Ilagan, R., Jain, D., Bertram, T. and Ludlow, J.:Organ Specific Regenerative Markers in Peri-Organ Adipose: Kidney.,Lipids Health Dis10, 171, 2011大鼠大鼠, E18NeuronsPapain: 0.05%NeurobasalGiacomello, M., Girardi, S., Scorzeto, M., Peruffo, A., Maschietto, M., Cozzi, B. and Vassanelli, S.:Stimulation of Ca?+ Signals in Neurons by Electrically Coupled Electrolyte-Oxide-Semiconductor Capacitors.,J Neurosci Methods 198, 1, 2011大鼠大鼠, SD, 初生Brown adipocytesCollagenase Type 4: 0.1%PBSLiu, Z., Wang, H., Zhang, Y., Zhou, J., Lin, Q., Wang, Y., Duan, C., Wu, K. and Wang, C.:Efficient Isolation of Cardiac Stem Cells from Brown Adipose.,J Biomed Biotechnol2010, 104296, 2010Neutral Protease: 0.1%Trypsin: 0.05%大鼠大鼠Adipose Derived StemCollagenase Type 1: 0.1%DMEMWei, X., Du, Z., Zhao, L., Feng, D., Wei, G., He, Y., Tan, J., Lee, W., Hampel, H., Dodel, R., Johnstone, B., March, K., Farlow, M. and Du, Y.:IFATS Collection: The Conditioned Media of Adipose Stromal Cells Protect Against Hypoxia-Ischemia-Induced Brain Damage In Neonatal Rats.,Stem Cells 27, 478, 2009大鼠大鼠Adipose tissue-derived stemCollagenase Type 2: 0.075%DMEMTomiyama, K., Murase, N., Stolz, D., Toyokawa, H., O Donnell, D., Smith, D., Dudas, J., Rubin, J. and Marra, K.:Characterization of Transplanted Green Fluorescent Protein+ Bone Marrow Cells Into Adipose Tissue.,Stem Cells 26, 330, 2008大鼠大鼠, 大鼠肉瘤, 4 周AdipocytesCollagenase: 0.2%Ham s F12Aoki, S., Toda, S., Sakemi, T., and Sugihara, H.:Coculture of Endothelial Cells and Mature Adipocytes Actively Promotes Immature Preadipocyte Development In Vitro,Cell Struct Funct 28, 55, 2003大鼠大鼠, SDAdipocytesCollagenase Type 1: 0.2%KRHBMora, S., Yang, C., Ryder, J., Boeglin, D and Pessin, J:The MEF2A and MEF2D Isoforms are Differentially Regulated in Muscle and Adipose Tissue during States of Insulin Deficiency,Endocrinology 142, 1999, 2001大鼠大鼠, SD, 雄性, 4-7周Brown adipocytesDeoxyribonuclease I: 0.5%DMEMOmatsu-Kanbe, M., and Matsuura, H.:Inhibition of Store-operated Ca2+ Entry by Extracellular ATP in Rat Brown Adipocytes,J Physiol521 (3), 601, 1999大鼠大鼠AdipocytesCollagenase Type 2: 0.2%DMEM /F-12Serrero, G: Primary Culture in Defined Medium of Adipocyte Precursors,Cell Tissue Culture: Laboratory Procedures Vol. 1,Doyle, A., Griffiths, J., and Newell, D., John Wiley and Sons, Ltd., 11B:6.1, 1995大鼠大鼠, SD, 雄性, 130-160 克AdiposeCollagenase: 0.3%Kreb s-Ringer bicarbonate buffer modifiedCharron, M.J. and Kahn, B.B.:Divergent Molecular Mechanisms for Insulin-Resistant Glucose Transport in Muscle and Adipose CellsIn Vivo,J Biol Chem265, 7994, 1990Epididymal fat pads大鼠大鼠, 3 天PreadipocytesCollagenase Type 3: 0.10%Parker Medium 199Gaben-Cogneville, A., Poussin, B., Chamblier, M., Forgue-Fafitte, M., and Rosselin, G.:Development of Insulin and Epidermal Growth Factor Receptors During the Differentiation of Rat Preadipocytes in Primary Culture,Biochim Biophys Acta968, 231, 1988大鼠大鼠, SD, 不同的重量和年龄Brown adipocytesCollagenase: 0.2%Krebs Ringer bicarbonate bufferWoodward, Julie A. and Saggerson, E.:Effect of Adenosine Deaminase, N6-Phenylisopropyladenosine and Hypothroidism on the Responsiveness of Rat Brown Adipocytes to Noradrenaline,Biochem J238, 395, 1986Interscapular cervical depotsSoybean Trypsin Inhibitor: 0.3%大鼠大鼠, CD, 雄性, 150-200克AdipocytesCollagenase: 0.1%Krebs Ringer bicarbonate bufferPessin, J.E., Gitomer, W., Oka, Y., Oppenheimer, C.L., and Czech, M.P.:?-Adrenergic Regulation of Insulin and Epidermal Growth Factor Receptors in Rat Adipocytes,J Biol Chem258, 7386, 1983Epididymal fat pads大鼠大鼠,大鼠肉瘤, 白化病, 雄性, 100-140 gAdipocytesCollagenase: 0.3%Kreb s RingerGreen, A. and Newsholme, E.:Sensitivity of Glucose Uptake and Lipolysis of White Adipocytes of the Rat to Insulin and Effects of Some Metabolites,Biochem J180, 365, 1979Epididymal-fat pads大鼠大鼠, 菲舍尔, 344, 雄性, 9 - 13 周White fatCollagenase: 0.3%Kreb s Ringer bicarbonate bufferStiles, J.W., Francendese, A.A. and Masoro, E.J.:Influence of Age on Size and Number of Fat Cells in the Epididymal Depot,Am J Physiol 229, 1561, 1975大鼠大鼠, 大鼠肉瘤, 雄性, 5 周-16 个月White fatCollagenase: 0.05%Kreb s Ringer bicarbonate bufferDiGirolamo, M., Mendlinger, S., and Fertig, J.W.:A Simple Method to Determine Fat Cell Size and Number in Four Mammalian Species,Am J Physiol 221, 850, 1971大鼠大鼠 (CFE), 白化病, 雌性Brown fatCollagenase Type 1: 0.10%Bicarbonate bufferFain, J., Reed, N., and Saperstein, R.:Isolation and Metabolism of Brown Fat Cells,J Biol Chem242, 1887, 1967大鼠大鼠, SD, 雄性, 160-210 克FatCollagenase: 0.3%Albumin-bicarbonate bufferRodbell, M.:Metabolism of Isolated Fat Cells. I. Effects of Hormones on Glucose Metabolism and Lipolysis,J Biol Chem239 (2), 375, 1964我们公司zui大优势是强大的采购,1:基本什么都能进口,
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